What do we mean by replications in DNA?
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- Fahad Hanif
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Many enzymes are necessary to successfully replicate DNA. First topoisomerase is used to unwind the DNA strand. By cutting one strand the tension in the supercoil is released. Next helicase is used to prevent the strand from recoiling. DNA polymerase III then travels across the strand, from the five carbon (5', or the fifth carbon on the phosphate group) to the third carbon (3', or the third carbon on the phosphate group). DNA polymerase III places the complementary nucleotide on the strand. So adenine would be put opposite of thymine (and the reverse), and guanine would be opposite of cytosine (and the reverse). DNA polymerase also needs a primer to begin attaching the nucleotides on the strand. Primase acts as the primer for DNA polymerase. Primase attaches a small RNA primer so that DNA polymerase can begin working. The RNA primer is removed by RNase H and DNA polymerase I fills the spot.
this is the procedure
Many enzymes are necessary to successfully replicate DNA. First topoisomerase is used to unwind the DNA strand. By cutting one strand the tension in the supercoil is released. Next helicase is used to prevent the strand from recoiling. DNA polymerase III then travels across the strand, from the five carbon (5', or the fifth carbon on the phosphate group) to the third carbon (3', or the third carbon on the phosphate group). DNA polymerase III places the complementary nucleotide on the strand. So adenine would be put opposite of thymine (and the reverse), and guanine would be opposite of cytosine (and the reverse). DNA polymerase also needs a primer to begin attaching the nucleotides on the strand. Primase acts as the primer for DNA polymerase. Primase attaches a small RNA primer so that DNA polymerase can begin working. The RNA primer is removed by RNase H and DNA polymerase I fills the spo
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